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One milliliter of culture was then back-diluted again into each of two flasks containing (1) 49 ml of DRM supplemented with 50 µg ml −1 carbenicillin, 50 µg ml −1 spectinomycin, 20 µM arabinose and 20 µg ml −1 chloramphenicol or (2) 49 ml of DRM supplemented with 50 µg ml −1 carbenicillin, 50 µg ml −1 spectinomycin and 20 µM arabinose
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