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Subsequently, the ATP6AP1L coding sequence was amplified from the cDNA of T47D cells and inserted into the FastDigest KpnI/FastDigest XbaI (Thermo Fisher) linearized pcDNA3.1-GST DAF and pcDNA3.1-GST CEAM7 vectors using T4 DNA ligase (EL0011, Thermo Fisher)
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This analysis revealed 294 shared substrates, 34 of which, including caspase-1, were predicted to be cleaved by X(PC) within 30 amino acids of a caspase cleavage site and in a flexible region, as determined by AlphaFold structural models (Fig
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