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Ambr ® 250 HT reactors (Sartorius AG, Göttingen, Germany) with 1 g/L Cytodex 1 microcarriers were operated for oncolytic virus production similar to a published method ( ® 250 HT bioreactors underwent an 80% medium exchange into WMEM without serum for a final concentration of 2% (v/v) BCS
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