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Cloning, expression and identification of rSmGST The first Spirometra GST gene deposited in GeneBank (AEI16476.1) was amplified by PCR with specific primers carrying BamHI and PstI restriction enzyme sites (underlined) (forward, 5′-AT GGATCC ATGGGTTC GCTCCCGGTTC-3′, and reverse, 5′-AT CTGCAG CTAAGCATCACCACGCCAG-3′), and the cycling protocol was as follows: 30 cycles of 95°C for 50 s, 60°C for 50 s and 72°C for 50 s
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